《植物生理学报》 2017, 53(1): 1-8

bHLH转录因子在茉莉酸信号诱导植物次生产物合成中的作用及分子机制

李欣¹, 李影¹, 曲子越¹, 孙璐¹, 王思瑶¹, 詹亚光^1,2, 尹静^1,2,*

东北林业大学¹生命科学学院, ²林学院林木遗传育种与生物技术国家重点实验室, 哈尔滨150040

通信作者：尹静；E-mail: yinjing20135@163.com

摘　要：

在茉莉酸(jasmonic acid, JA)信号途径中, 植物通过转录激活子和转录抑制子之间的合理协调来调控适当的防御反应及生长发育过程, 从而适应多变的环境, 以刺激次生产物有效合成, 其中bHLH (basic helix-loop-helix)转录因子在响应JA信号诱导植物次生产物萜类合成中起重要调控作用。本文结合植物中bHLH转录因子的结构特点和JA信号在萜类物质合成中的作用, 系统地阐述了bHLH转录因子在响应JA信号诱导萜类合成中的调控作用及分子机制, 并探讨了bHLH类转录因子与MYB类转录因子互作参与次生代谢调控的过程。本综述为进一步深入揭示bHLH在植物萜类合成代谢调控网络中的分子功能和药用植物活性成分代谢途径的遗传改良提供理论参考。

关键词：bHLH; 茉莉酸; 萜类; 调控机制

收稿：2016-09-22   修定：2016-12-02

资助：国家自然科学基金(31570589)、中央高校基本科研业务费(257-2015DY01)和国家基础科学人才培养基金(J1210053)。

The molecular mechanism and the function of bHLH regulating jasmonic acidmediated secondary metabolites synthesis

LI Xin¹, LI Ying¹, QU Zi-Yue¹, SUN Lu¹, WANG Si-Yao¹, ZHAN Ya-Guang^1,2, YIN Jing^1,2,*

¹College of Life Sciences; ²State Key Laboratory of Tree Genetics Breeding, School of Forestry, Northeast Forestry University, Harbin 150040, China

Corresponding author: YIN Jing; E-mail: yinjing20135@163.com

Abstract:

In jasmonic acid (JA) signaling pathways, the proper defense reaction and the development process of the plant were regulated through transcription activation factor and transcription inhibition factor, in order to adapt to the changing environment and to stimulate synthesis of secondary products. The bHLH transcription factor plays an important role in JA inducing synthesis of terpenoids. Combining with structure characteristics of the bHLH transcription factors and the role of jasmonate-mediated in terpenoids synthesis, the molecular mechanism of bHLH regulated JA-mediated terpenoids synthesis were expound systematically, and interaction of the bHLH transcription factor with MYB transcription factors regulation participated in the process of secondary metabolism were discussed in this paper. This review deeply reveals the molecular function of bHLH in plant terpenes synthesis, and provides theoretical basis of genetic improvement in metabolic pathways of active components from medicine plant.

Key words: bHLH; jasmonic acid; terpenoids; regulatory mechanism